Dietary l-Arginine Reduces the Progression of Atherosclerosis in Cholesterol-Fed Rabbits

Comparison With Lovastatin

From: http://circ.ahajournals.org/content/96/4/1282.long

1. Rainer H. Böger, MD;
2. Stefanie M. Bode-Böger, MD;
3. Ralf P. Brandes, MD;
4. Laddaval Phivthong-ngam, PhD;
5. Michael Böhme;
6. Reinhold Nafe, MD;
7. Andreas Mügge, MD;
8. Jürgen C. Frölich, MD

+ Author Affiliations

1. From the Institute of Clinical Pharmacology (R.H.B., S.M.B.-B., L.P., M.B., J.C.F.) and Departments of Cardiology (R.P.B., A.M.) and Pathology (R.N.), Medical School, Hannover, Germany.

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Abstract

Background We investigated whether l-arginine induces regression of preexisting atheromatous lesions and reversal of endothelial dysfunction in hypercholesterolemic rabbits, whether similar effects can be obtained by cholesterol-lowering therapy with lovastatin, and which mechanism leads to these effects.

Methods and Results Rabbits were fed 1% cholesterol for 4 weeks and 0.5% cholesterol for an additional 12 weeks. Two groups of cholesterol-fed rabbits were treated with l-arginine (2.0% in drinking water) or lovastatin (10 mg/d) during weeks 5 through 16. Systemic nitric oxide (NO) formation was assessed as the urinary excretion rates of nitrate and cGMP in weekly intervals. Cholesterol feeding progressively reduced urinary nitrate excretion to ≈40% of baseline (P<.05) and increased plasma concentrations of asymmetrical dimethylarginine (ADMA), an endogenous NO synthesis inhibitor. Dietaryl-arginine reversed the reduction in plasma l-arginine/ADMA ratio and partly restored urinary excretion of nitrate and cGMP (each P<.05 vs cholesterol) but did not change plasma cholesterol levels. l-Arginine completely blocked the progression of carotid intimal plaques, reduced aortic intimal thickening, and preserved endothelium-dependent vasodilator function. Lovastatin treatment reduced plasma cholesterol by 32% but did not improve urinary nitrate or cGMP excretion or endothelium-dependent vasodilation. Lovastatin had a weaker inhibitory effect on carotid plaque formation and aortic intimal thickening than l-arginine. l-Arginine inhibited but lovastatin potentiated superoxide radical generation in the atherosclerotic vascular wall.

Conclusions Dietary l-arginine improves NO-dependent vasodilator function in cholesterol-fed rabbits and completely blocks the progression of plaques via restoration of NO synthase substrate availability and reduction of vascular oxidative stress. Lovastatin treatment has a weaker inhibitory effect on the progression of atherosclerosis and no effect on vascular NO elaboration, which may be due to its stimulatory effect on vascular superoxide radical generation.